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Real-time fluorescence trajectories of two different S65T molecules. (a and c) Total fluorescence time trace, showing mainly background signal with occasional GFP fluorescence bursts. (b and d) Magnification of the fluorescence bursts, with ≈2 × 104 counts in a 0.5-s burst (b), and ≈5 × 103 counts in a 0.2-s burst (d).
