TABLE 1.
Amplification primers and molecular beacon probes used in this study
| Oligonucleotide | Sequence | Positiona |
|---|---|---|
| Primers | ||
| grlA205 (Forward) | 5′-GAT GTT ATT GGT CAA TAT CAT CCA-3′ | 205-228 |
| grlA396 (Reverse) | 5′-AAG AAA CTG TCT CTT TAT TAA TAT CAC GT-3′ | 396-424 |
| Probesb | ||
| S80-FAM | 5′ (FAM)-CAG CGT ACA CTG AGG AGT CTC CGC TG-3′ (DABCYL) | 233-248 |
| E84-FAM | 5′ (FAM)-CGC GAA GTG TAC GAA GCA ATG GTC GCG-3′ (DABCYL) | 243-259 |
| Sau-TET | 5′ (TET)-CCG GGT ATC GAT AAT GAT CCG CCA GCC CGG-3′ (DABCYL) | 324-343 |
a
Nucleotide positions in the grlA gene (start codon, 1 to 3) from GenBank accession no. D67075.
b
The underlined sequences constitute the stem of each molecular beacon. FAM, 6-carboxylfluorescein; TET, tetrachloro-6-carboxylfluorescein.