TABLE 2.
Evaluation of the real-time PCR assay for the detection of fluoroquinolone-resistant Staphylococcus aureus using a variety of bacteria
| Bacterial species (strain number) | Fluorescent signal with the indicated test and probea
|
|||
|---|---|---|---|---|
| PCR no. 1
|
PCR no. 2
|
|||
| Sau-TET | S80-FAM | Sau-TET | E84-FAM | |
| Abiotrophia defectiva (ATCC 49176) | − | − | − | − |
| Bacillus cereus (ATCC 13472) | − | − | − | − |
| Bacillus mycoides (ATCC 10206) | − | − | − | − |
| Enterococcus faecalis (ATCC 29212) | − | − | − | − |
| Enterococcus faecalis (ATCC 19433) | − | − | − | − |
| Enterococcus flavescens (ATCC 49996) | − | − | − | − |
| Granulicatella adiacens (ATCC 49175) | − | − | − | − |
| Lactococcus lactis (ATCC 11454) | − | − | − | − |
| Listeria innocua (ATCC 33090) | − | − | − | − |
| Listeria monocytogenes (ATCC 15313) | − | − | − | − |
| Staphylococcus auricularis (ATCC 33753) | − | − | − | − |
| Staphylococcus capitis subsp. urealyticus (ATCC 49326) | − | − | − | − |
| Staphylococcus carnosus (ATCC 51365) | − | − | − | − |
| Staphylococcus chromogenes (ATCC 43764) | − | − | − | − |
| Staphylococcus epidermidis (ATCC 35983) | − | − | − | − |
| Staphylococcus epidermidis (ATCC 35984) | − | − | − | − |
| Staphylococcus gallinarum (ATCC 35539) | − | − | − | − |
| Staphylococcus haemolyticus (ATCC 29970) | − | − | − | − |
| Staphylococcus haemolyticus (LSPQ 2514) | − | − | − | − |
| Staphylococcus hominis subsp. hominis (ATCC 27844) | − | − | − | − |
| Staphylococcus lentus (ATCC 29070) | − | − | − | − |
| Staphylococcus lugdunensis (ATCC 43809) | − | − | − | − |
| Staphylococcus saccharolyticus (ATCC 14953) | − | − | − | − |
| Staphylococcus saprophyticus (ATCC 15305) | − | − | − | − |
| Staphylococcus saprophyticus (ATCC 35552) | − | − | − | − |
| Staphylococcus saprophyticus (ATCC 43867) | − | − | − | − |
| Staphylococcus simulans (ATCC 27848) | − | − | − | − |
| Staphylococcus warneri (ATCC 27836) | − | − | − | − |
| Staphylococcus xylosus (ATCC 29971) | − | − | − | − |
| Streptococcus agalactiae (ATCC 12400) | − | − | − | − |
| Streptococcus pneumoniae (ATCC 49619) | − | − | − | − |
| S. aureus (ATCC 25923)b | + | + | + | + |
| S. aureus (ATCC 29213)b | + | + | + | + |
| S. aureus (CCRI-2024)b | + | − | + | + |
| S. aureus (9191)b | + | − | + | + |
| S. aureus (9299)b | + | − | + | + |
| S. aureus (9015)b | + | + | + | − |
| S. aureus (9283)b | + | + | + | − |
| S. aureus (CCRI-12876)b | + | − | + | − |
a
All PCR tests were performed using 0.1 ng of purified genomic DNA.
b
These strains were chosen to represent different mutations at codons 80 and 84 of grlA. The quinolone resistance-determining regions of grlA from these strains were sequenced in this study. S. aureus strains ATCC 25923 and ATCC 29213 have no mutation at codons 80 and 84; strains CCRI-2024, 9191, and 9299 have a Ser-80-to-Phe (TCC→TTC) mutation; strain 9015 has a Glu-84-to-Gly (GAA→GGA) mutation; strain 9283 has a Glu-84-to-Lys (GAA→AAA) mutation; and strain CCRI-12876 has both Ser-80-to-Tyr (TCC→TAC) and Glu-84 to Gly (GAA→GGA) mutations.