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Amplification of rpoB DNAs (458 bp) from Helicobacter species (A) and other bacteria (B). PCR products were electrophoresed on a 1.2% agarose gel. Lane M in both panels, φ X174 RF DNA/HaeIII digest. (A) Lanes: 1 to 6, H. pylori isolates; 7, H. felis; 8, H. cinaedi; 9, H. mustelae; and 10, H. hepaticus. (B) Lanes: H, H. pylori isolate; 2, E. coli, 3, C. diphtheriae; 4, S. aureus; 5, B. subtilis; 6, M. fortuitum; 7, B. catarrhalis; 8, H. influenzae; 9, N. sicca; and 10, E. faecalis.
