TABLE 1.
Multiplex primers for CMV gBs
| Amplification reaction and primer | Polaritya | Position (nucleotides)b | Sequence (5′→3′) |
|---|---|---|---|
| Primary | |||
| CMVQ1+ | HS5GLYBG+ | 868-885 | TTTGGAGAAAACGCCGAC |
| CMVQ1- | HS5GLYBG- | 1619-1597 | CGCGCGGCAATCGGTTTGTTGTA |
| Nestedc | |||
| CMVGT1+ | HS5GLYBG+ | 1111-1130 | ATGACCGCCACTTTCTTATC |
| CMVGT2+ | HEHCMVGB+ | 1074-1096 | TTCCGACTTTGGAAGACCCAACG |
| CMVGT3+ | HS5GLYBM+ | 1341-1359 | TAGCTCCGGTGTGAACTCC |
| CMVGT4+ | HS5GLYBD+ | 1057-1082 | ACCATTCGTTCCGAAGCCGAGGAGTCA |
| CMVGT5+ | AF043721+ | 307-325 | TACCCTATCGCTGGAGAAC |
| CMVQ2- | HS5GLYBG- | 1531-1513 | GTTGATCCACRCACCAGGC |
a
+, forward; −, reverse.
b
The numbering positions for HS5GLYBG (6), HEHCMVGB (7), HS5GLYBM (6), HS5GLYBD (6), and AF043721 (21) are as described previously.
c
The reverse primer was shared by all CMV gBs.