TABLE 2.
Evaluation of HCV RNA linearitya of the b-DNA assay on liver biopsy samples
| Dilution | HCV RNA linearity
|
||||
|---|---|---|---|---|---|
| Expected value
|
Observed value
|
Δlog10 | |||
| IU/μg | IU log10 | IU/μg | IU log10 | ||
| 96,154 | 4.98 | 102,596 | 5.01 | 0.03 | |
| 1:2 | 48,077 | 4.68 | 56,127 | 4.75 | 0.07 |
| 1:4 | 24,038 | 4.38 | 28,587 | 4.46 | 0.08 |
| 1:8 | 12,019 | 4.08 | 14,858 | 4.17 | 0.09 |
| 1:16 | 6,010 | 3.78 | 6,452 | 3.81 | 0.03 |
| 1:32 | 3,005 | 3.48 | 2,671 | 3.43 | −0.05 |
| 1:64 | 1,502 | 3.18 | 791 | 2.90 | −0.28 |
| 1:128 | 751 | 2.88 | 1,057 | 3.02 | 0.15 |
a
The Spearman correlation coefficient between expected and observed values was 0.98 (P < 0.01).