TABLE 1.
Comparative specificity of DNA detection by using the C. albicans species-specific and all-Candida genus-specific fluorescent probes
| Genomic DNAa |
CTb
|
|
|---|---|---|
| CA-FAM | All-CAN-TET | |
| S. cerevisiae | − | 16.91 ± 0.14 |
| C. parapsilosis | − | 20.22 ± 0.20 |
| C. albicans | 17.54 ± 0.11 | 20.23 ± 0.27 |
| A. nidulans | − | 20.31 ± 0.26 |
| Penicillium spp. | − | 20.92 ± 0.07 |
| C. krusei | − | 21.28 ± 0.10 |
| C. guilliermondii | − | 21.29 ± 0.18 |
| C. glabrata | − | 21.57 ± 0.03 |
| C. tropicalis | − | 21.64 ± 0.29 |
| C. norvegensis | − | 21.67 ± 0.21 |
| A. flavus | − | 21.84 ± 0.13 |
| A. fumigatus | − | 22.73 ± 0.24 |
| Curvularia spp. | − | 22.74 ± 0.01 |
| Fusarium spp. | − | 23.20 ± 0.19 |
| Alternaria spp. | − | 24.05 ± 0.07 |
| H. werneckii | − | 24.45 ± 0.16 |
| P. verrucosa | − | 24.54 ± 0.21 |
| E. purpurescens | − | 24.77 ± 0.16 |
| A. niger | − | 25.30 ± 0.14 |
| Auxarthron spp. | − | 26.20 ± 0.11 |
| C. neoformans | − | 26.36 ± 0.18 |
| S. apiospermum | − | 26.39 ± 0.22 |
| Chaetomium spp. | − | 32.19 ± 0.01 |
| Klebsiella spp. | − | − |
| H. influenzae | − | − |
| M. morganii | − | − |
| S. maltophilia | − | − |
| P. mirabilis | − | − |
| P. aeruginosa | − | − |
| Human lymphocyte line | − | − |
| Cytomegalovirus | − | − |
a
One nanogram of purified DNA was analyzed.
b
Values are expressed as means ± standard deviations. Data are from triplicate samples from two to three experiments. A negative value was assigned for all CT values equal to 40.