FIG. 2.

Evaluation of various mixtures of Fab fusion proteins. Mix A contains B6Fabenv13 and B6Fabenv24 each at 10 μg/ml; mix B contains B6Fabenv13, B6Fabenv24, 10F7 Fabenv13 and 10F7Fabenv24 each at 10 μg/ml; and mix C contains A41Fabp246 (15 μg/ml) in addition to the components of mix B. All three mixtures were tested with the samples of panel PRB202 by a slide assay. Twenty microliters of a 1:1 mix of a serum sample from panel PRB202 and RBCs from an individual with type O RhD-negative blood was added to 20 μl of each reagent on a glass slide, and the agglutination reaction was read visually on an arbitrary scale of 0 to 4, with 0 indicating no visible agglutination and 4 indicating strong agglutination with large clumps.