TABLE 4.
Evaluation of NEVA HIV with clinical samples from various hospitals in Indiaa
| Center | Nature of samples | No. of samples | Sensitivity | Specificity |
|---|---|---|---|---|
| Christian Medical College, Vellore | Serum and 26 whole-blood samplesb | 326 | 100c (174/174)d | 99.3c (151/152) |
| Institute of Pathology, New Delhi | Serum | 55 | 100 (5/5) | 100 (50/50) |
| Post Graduate Institute of Medical Research, Chandigarh | Serum | 132 | 100e (80/80) | 96.2e (50/52) |
| National AIDS Research Institute, Pune | Serum and 6 clotted blood samplesf | 269 | 100g (49/49) | 97.8g (215/220) |
| Safdarjang Hospital, New Delhi | Random whole-blood samples | 331 | 100h (1/1) | 98.8h (326/330) |
| Whole-blood samples from a sexually transmitted disease clinic | 164 | 100h (9/9) | 99.4h (155/156) | |
| All samples | 1,277 | 100 (318/318) | 98.6 (947/960) |
The serum samples were evaluated after they were mixed with type O negative RBCs (Table 1). Whole-blood samples were used directly (20 μl/spot). For clotted samples (clotted within 12 h of collection), the clot was broken with a plastic dropper, and cells from the clot mixed with plasma were used for the assay (20 μl/spot).
Including 5 serum samples from monkeys, 9 human T-cell leukemia type 1-positive samples, 21 samples from a sexually transmitted disease clinic, 13 samples from patients with typhoid fever, 3 samples from patients with tuberculosis, 8 samples from patients with syphilis, and 4 samples positive for HIV-2 only.
In comparison to center's data, which were obtained by two EIAs and Western blotting.
The values in parentheses indicate the number of samples positive/total number of samples tested.
In comparison to the center's data, which were obtained by two or three EIAs.
Including 116 samples from patients with tuberculosis, 34 samples from the Venereal Disease Research Laboratory, and 113 random serum samples.
In comparison to the center's data, which were obtained by EIA.
In comparison to data obtained by EIA.