TABLE 1.
Reproducibility of sequencing of replicate PCR products from single RNA extracts from 46 samples
| Sequence comparison of PCR productsa | Nucleic acid resultsb
|
Amino acid resultsb
|
||||
|---|---|---|---|---|---|---|
| Concordance (%)c | Total no. of discordances | No. (%) of partial discordances | Concordance (%)d | Total no. of discordances | No. (%) of partial discordances | |
| A1 vs B1 | 99.4 | 440 | 330 (75) | 99.3 | 170 | 130 (76.4) |
| A1 vs A2 | 99.8 | 127 | 125 (98.4) | 99.8 | 51 | 50 (98) |
| B1 vs B2 | 99.8 | 120 | 118 (98.3) | 99.8 | 46 | 43 (93.5) |
| A2 vs A3 | 99.9 | 92 | 89 (96.7) | 99.8 | 45 | 39 (86.7) |
| B2 vs B3 | 99.9 | 100 | 98 (98) | 99.8 | 56 | 56 (100) |
a
Identified according to Fig. 1. The total numbers of nucleotides and amino acids analyzed in each arm (A1, A2, A3, B1, B2, and B3) were 68,862 and 22,954, respectively.
b
Partial and complete discordances are defined in Materials and Methods.
c
Inter-rater concordance, >99.9%.
d
Inter-rater concordance, 99.9%.