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Analysis of the reconstituted nucleosomes by AFM. Nucleosomes were reconstituted with pUC-α11mer DNA and native core histones (A, C, and E) or His6CENP-A/core histones with EK digestion (B, D, and F) and observed by AFM. (A and B) Nucleosomes formed on circular DNA. (Bar indicates 100 nm.) (C and D) Histogram of the number of nucleosomes in each volume class. (E and F) Plot of the contour length of each circular DNA molecule against the number of nucleosomes on it.
