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. 2000 Jun 6;97(13):7266–7271. doi: 10.1073/pnas.130189697

Table 1.

Results of purification of CENP-A from HeLa cells (∼1 × 1010)

Fraction Total protein Yield, % CENP-A, μg Purity, %
0.6 M NaCl nuclear pellet 33
Chromatin protein 120 mg 90 30 0.025
1st HPLC 360 μg 45 15 4.2
2nd HPLC 90 μg 30 10 11
SDS/PAGE/electroelution 5 μg 15 5 >95