FIG. 2.

Sensitivity and specificity of the LACV amplicon. (A) Amplification blot of the LACV RNA standard from 10⁷ to 10¹ molecules (from left to right: 10⁷, blue; 10⁶, green; 10⁵, red; 10⁴, black; 10³, red; 10², green; and 10², blue). (B) The LACV species-specific assay was tested with 10⁷ copies of the standard RNA of all of the other Orthobunyavirus species. The LACV amplicon showed minor cross amplification of 10⁷ copies of the GROV standard RNA (red curve) but no cross amplification of the standard RNA of any other strain (all other colors).