FIG. 3.

Detection of OROV in patient sera. (A) OROV RNA standard range from 10⁷ to 10¹ molecules (as established on the SmartCycler). (B) Results are given as the number of copies of viral RNA detected per milliliter of serum obtained from each of 28 patients sampled on days 1 to 5 after onset of disease. Two patient samples drawn on days 2 and 3 after onset of disease tested negative. RNA was Trizol extracted from 125 μl of serum. OROV infection had been previously confirmed by virus isolation.