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Distribution of ALL-1 and huASH1 speckles in nuclei of human foreskin fibroblasts (HFF). Cells were double-stained with rabbit and guinea pig Ab against ALL-1 and huASH1, respectively. The relative distributions were examined by fluorescence ratio imaging using digital fluorescence microscopy. The localization of the two proteins is largely mutually exclusive as evidenced by the very small number of yellow spots representing colocalization.
