FIG. 3.

Demonstration of a linear relation between DNA concentration and peak heights. Peak-height ratios (mutant/wild type) were plotted versus the corresponding DNA concentration ratios for the four sequencing primers: 74F (A), 74R (B), 184F (C), and 184R (D). Each plot shows 15 datum points (ratios of peak heights) obtained from three independent sequencing runs. A linear regression analysis was performed for each of the three independent runs (see Results). The combined linear regression correlation coefficients (R²) for primers 74F, 74R, 184F, and 184R were 0.994, 0.997, 0.976, and 0.998, respectively.