TABLE 1.
PCR primers for amplification of cagA and vacA sequences
| Gene and DNA region amplified | Primer | Primer sequence (5′→3′)a | PCR product size (bp) | Reference |
|---|---|---|---|---|
| cagA | CAGAF | GATAACAGGCAAGCTTTTGAGG | 349 | 11 |
| CAGAR | CTGCAAAAGATTGTTTGGCAGA | 11 | ||
| cagA | CAGA5F | GGCAATGGTGGTCCTGGAGCTAGGC | 325 | 6 |
| CAGA5R | GGAAATCTTTAATCTCAGTTCGG | 6 | ||
| cag empty site | Luni1 | ACATTTTGGCTAAATAAACGCTG | 550 | 6 |
| R5280 | GGTTGCACGCATTTTCCCTTAATC | 6 | ||
| vacA s1 | VA1-F | ATGGAAATACAACAAACACAC | 259 | 1 |
| VA1-R | CTGCTTGAATGCGCCAAAC | 1 | ||
| vacA s2 | VA1-F | ATGGAAATACAACAAACACAC | 286 | 1 |
| VA1-R | CTGCTTGAATGCGCCAAAC | 1 | ||
| vacA s1a | S1A-Fb | TCTYGCTTTAGTAGGAGC | 212 | 9 |
| vacA s1b | SS3-Fb | AGCGCCATACCGCAAGAG | 187 | 1 |
| vacA m1 | VAG-F | CAATCTGTCCAATCAAGCGAG | 570 | 11 |
| VAG-R | GCGTCTAAATAATTCCAAGG | 11 | ||
| vacA m2 | VAG-F | CAATCTGTCCAATCAAGCGAG | 645 | 11 |
| VAG-R | GCGTCTAAATAATTCCAAGG | 11 |
a
Y = C + T.
b
Used with primer VA1-R.