Figure 5.

(A) dp53 RNA is expressed throughout development. Total RNA from the indicated developmental stages was subjected to an RNase-protection assay using a dp53-specific probe. Note the elevated levels of dp53 RNA in females and early embryos. Tubulin RNA was used as loading control. (B–F) In situ hybridization was performed on wild-type embryos using a dp53-specific RNA probe. (B) Stage 10 egg chamber. Note presence of dp53 RNA in the nurse cells (nc) of the egg chamber. No signal was observed in the somatic follicle cells (fc). o, oocyte. (C) Blastoderm embryo probed with antisense RNA; the signal inside the egg indicates a maternal component of dp53 RNA. (D and E) Embryos before and after germ band retraction, showing a ubiquitous expression of dp53. (E) Embryo probed with sense RNA as a control for the specificity of the probe.