Figure 5.

Phenotypic Effects of Reduced CUC3 Expression.

(A) Semiquantitative RT-PCR of CUC3 expression in homozygous WET368A and WET368B plants and in wild-type Ler. ROC5 expression was used to normalize CUC3 expression levels.

(B) and (C) Relative abundance of seedling phenotypes among the progeny of plants with the indicated genotypes of CUC1, CUC2, and CUC3. + indicates wild-type alleles, cuc1 and cuc2 indicate mutant alleles, cuc3-1 indicates the CUC3 hypomorph allele caused by the WET368 Ds insertion, and cuc3-2 indicates the CUC3 mutant allele caused by the T-DNA insertion in exon 2. Numerals beside the bars indicate the total number of seedlings analyzed. WS, wild-type Wassilewskija.

(B) Percentages of heart-shaped seedlings.

(C) Percentages of cup-shaped cotyledon seedlings. f-cuc seedlings are shown in black, and s-cuc seedlings are shown in gray. Data labels indicate the percentages of f-cuc seedlings among the entire progeny.