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. 2003 Jul;15(7):1578–1590. doi: 10.1105/tpc.011890

Figure 2.

Figure 2.

AtPirin1 Amino Acid Sequence Analysis, AtPirin1 Gene Structure, and Locations of the T-DNA Insertions.

(A) Alignment of the deduced amino acid sequences of AtPirin1, AtPirin2, AtPirin3, AtPirin4, tomato pirin (TOMpirin), and human pirin (HUMpirin). The alignment was generated using the CLUSTAL X program with default parameters. Identical amino acids are highlighted with a black background, and similar residues are highlighted with a gray background. Regions corresponding to motifs 1 and 2 of the cupin domain are indicated. The single and double asterisks mark residues corresponding to the beginning of the longest and shortest GPA1-interacting AtPirin1 clones, respectively, identified in the two-hybrid screen. Arrows indicate the positions of T-DNA insertions in the atpirin1-1 and atpirin1-2 mutants.

(B) Scheme of the AtPirin1 gene. Relative positions of potential cis elements mentioned in the text are shown. The positions of the atpirin1-1 and atpirin1-2 T-DNA insertions are indicated. Boxes represent exons, and motifs 1 and 2 of the cupin domain are marked by vertical and horizontal hatching, respectively.