Figure 2.

pie1 Mutant Phenotype in Different Genetic Backgrounds.

(A) pie1-1 in the Ws background and wild-type Ws grown for 25 days in long days.

(B) Wild-type Col and pie1-2, pie1-3, and pie1-4 in the Col background grown in long days until flowering had initiated.

(C) The pleiotropic phenotype of Col pie1 alleles after the transition to flowering. A small fraction of pie1 mutant plants in Col showed some degree of primary inflorescence elongation (left); however, the majority of pie1 mutant plants displayed a more severe inhibition of primary inflorescence elongation and the development of numerous secondary inflorescences (middle), which led to a bushy phenotype in older plants (right). DAP, days after planting.

(D) The genetic background influences the pie1 phenotype. Shown are representative pie1-2 plants from the F2 population obtained from a cross between pie1-2 in Col and Ws. Approximately three-quarters of the pie1-2 mutant plants displayed the more normal phenotype (left), whereas approximately one-quarter of the pie1-2 mutant plants displayed the more pleiotropic phenotype (right) characteristic of Col pie1 alleles.

(E) Leaf phenotypes of pie1 mutants. Shown are the fifth rosette leaves from wild-type plants (Ws and Col) and pie1 mutants in the corresponding genetic backgrounds (pie1-1 and pie1-2, respectively) grown in short days.