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TGase-S forms large aggregates in cells. About 12 h after the transfection of NIH 3T3 cells with the indicated TGase and TGase-S constructs, the cells were fixed. (a) Immunofluorescence was performed on the samples by using Myc antibody. The top and side views of the transfectants are shown. (b) Immunofluorescence was also performed on samples by using Myc and β-COP antibodies and DAPI to detect transfectants, Golgi, and nuclei, respectively.
