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Rate constants and kinetically measured dissociation constant of IHF–DNA kinetics at 20°C
| Substrate | kon, M−1·s−1 | koff, s−1 | KD, nM |
|---|---|---|---|
| Intact | 5.2 × 108 | 0.013 | 0.025 |
| Nick A | 7.4 × 108 | 0.020 | 0.027 |
| Nick B | 5.0 × 108 | 0.029 | 0.058 |
| Long | 4.3 × 108 | 0.029 | 0.067 |
Association rate constant (kon), dissociation rate constant (koff), and kinetic dissociation constant (KD= koff/kon) for intact, nick A, nick B, and long substrates measured and calculated are summarized.
