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Design and functionality of CoMed™ vector system. The CoMed™ basic vector contains all E. coli elements for propagation in the E. coli system and a MCS for integration of ARS, rDNA, selection marker and expression cassette modules. For this purpose, ARS fragments are flanked by SacII and BcuI restriction sites, rDNA regions by BcuI and Eco47III restriction sites, selection markers by Eco47III and SalI restriction sites and promoter elements by SalI and ApaI restriction sites [38]).
