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. 2006 Nov 14;5:33. doi: 10.1186/1475-2859-5-33

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Copyright © 2006 Steinborn et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Physical map of the expression/integration vector pAL-HPH-TEF-GFP used in this study. The vector contains the 25S rDNA sequence of A. adeninivorans (rDNA, white box) and an expression cassette for the E. coli-derived hph gene as selection marker in the order A. adeninivorans-derived TEF1 promoter (TEF1 pro,grey segment), the hph-coding sequence (HPH, grey segment), S. cerevisiae-derived PHO5 terminator (PHO5 ter, black bar). The vector further contains a second expression cassette with TEF1 promoter – GFP ORF – PHO5 terminator elements. The vector contains further an unique BglII site within the rDNA sequence for linearization. (B) Detection of recombinant GFP-producing yeast cells by fluorescence microscopy. Transformants were cultured for 48 h in YEPD medium at 30°C and subsequently used for fluorescence analysis. (I) transmission, (II) GFP-fluorescence.