Figure 2.

GLUT8 expression is detected in most tissue RNA. (A) Northern blot of total RNA from adult and fetal mouse tissue (15 μg) with a GLUT8 cDNA probe prepared as described in Methods. GLUT8 is present as a 2.1-kilobase band. E18, embryonic day 18. (B) Quantitation of RNA. The same blot was stripped and rehybridized to a mouse ribosomal protein S2 cDNA fragment that served as an internal control. The OD of each mRNA band was assessed with a Molecular Dynamics PhosphorImager and imagequant software (Molecular Dynamics) and was expressed as a percentage of the corresponding S2 message.