Figure 3.

GLUT8 detected in overexpression systems or endogenous mouse tissue. (A) Immunoprecipitation/immunoblot analysis of FLAG-GLUT8 overexpressed in COS-7 cells. Whole-cell lysates were made from COS-7 cells transfected either with vector only (Vector) or with a construct expressing GLUT8 tagged with the peptide tag FLAG (DYKDDDDK). All lysates were immunoprecipitated (IP) with anti-FLAGM2, and immunoblot (IB) analysis was performed with anti-FLAGM2, anti-GLUT8, and anti-GLUT8 + peptide. Immunoglobulin heavy chain (Ig HC) and immunoglobulin (Ig LC) are indicated by arrows. (B) GLUT8 protein is detected as an ≈37-kDa band in adult mouse brain (Br), heart (He), skeletal muscle (SkM), and blastocyst stage embryos (Blast). Each adult tissue lane contains 100 μg of protein. The blastocyst lane contain ≈279 blastocysts for a total of ≈7 μg of protein.