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. 2000 Jun 20;97(13):7313–7318. doi: 10.1073/pnas.97.13.7313

Figure 5.

Figure 5

GLUT8 is responsible for insulin-stimulated glucose uptake in the mouse blastocyst. (A) Confocal immunofluorescent labeling with GLUT8 antibody to determine the effect of treatment on GLUT8 expression. Embryos cultured in GLUT8 antisense demonstrate little or no GLUT protein expression compared with those cultured in sense. More than 15 blastocysts were evaluated for each group. (B) Insulin-stimulated glucose transport measured as pmol per embryo per 15 min or change in basal vs. insulin-stimulated 2-deoxyglucose taken up by individual blastocysts cultured in control medium (open bar), medium with 5 μM GLUT8 sense (black bar), or medium with 5 μM GLUT8 antisense (striped bar). Each bar represents three experiments totaling approximately 45 blastocysts. *, P < 0.001 by ANOVA with Fisher's test.