Figure 3.

Thin-layer chromatograms of stomatin and active stomatin fractions. Lane 1, crude stomatin (2 μl, 6 μg); lane 2, peak 1 (shown in Fig. 2A, 10 μl) obtained by HPLC separation of stomatin with H₂O as the mobile phase; lane 3, peak 2 (shown in Fig. 2A, 20 μl) obtained by HPLC separation of stomatin with H₂O as the mobile phase; lane 4, peak 3 (shown in Fig. 2A, 30 μl) obtained by HPLC separation of stomatin with H₂O as the mobile phase; lane 5, peak 4 from stomatin (shown in Fig. 2B, 10 μl) eluting at 100% acetonitrile; lane 6, peak eluting at 100% acetonitrile (10 μl) when the initial void-volume fraction was rechromatographed by using the acetonitrile gradient as the mobile phase; lane 7, eluted spot (5 μl) from TLC-purified peak 1 (Fig. 2A); lanes 8 and 9, commercially obtained hypoxanthine and uracil standards (25 nmol each) dissolved in H₂O. The major active spot is indicated by the arrow. A spot with lesser activity probably originating from dissociation of the major active component is indicated by the arrowhead.