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RNase III cleavage sites in the 3' strand of pre-23 S rRNA. 70 S ribosomes, 50 S subunits or in vitro transcribed 30 S pre-rRNA were incubated with or without RNase III (+ and - lanes respectively). The cleavage sites were determined by S1 nuclease mapping. Sequencing lanes A, C, G, and T were used to determine of exact length of protected 3' [32P]DNA. The positions of the RNase III cleavage and the mature 3' end of 23 S rRNA are indicated on the right.
