Figure 4.

Notum to wing transformations induced by interference with DER signaling are mediated by Wg. Early flip-out clones were identified by the expression of green fluorescent protein (green). (A and B) Flip-out clones that overexpressed DNRaf^3.1. Notum to wing transformation showing ectopic Vg expression (red). (C–F) Flip-out clones that overexpressed DNRaf^3.1 and DNWg. (C and D) No notum to wing transformations were found (data not shown) and no ectopic Vg expression was detected, despite the fact that the whole posterior compartment expresses both proteins. (E and F) Posterior to anterior transformation in the wing pouch is not abolished. Ci protein (red) is ectopically expressed in posterior territories (arrowhead).