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Representative pituitary sections of POMC mRNA expression in Crh+/+ (A, C, and E) and Crh−/− (B, D, and F) fetal mice from e16.5 (A and B), e17.5 (C and D), and e18.5 (E and F). Fresh-frozen midsagittal sections (oriented as depicted in G) were subjected to in situ hybridization by using a mouse POMC riboprobe. No difference was observed in POMC mRNA expression between the two genotypes at any age (n = 5 embryos/group).
