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. 2000 Jun 20;97(13):7342–7347. doi: 10.1073/pnas.97.13.7342

Figure 4.

Figure 4

DNA-binding studies of BEN. (A) EMSA of the binding of in vitro translated BEN protein to the EFG probe. All lanes contain labeled EFG oligonucleotide and in vitro translated BEN protein. Lane 1, no competitor; lane 2 to 5 have 5-, 10-, 20-, and 40-fold excess of cold EFG oligonucleotide. (B) Southwestern blot analysis of BEN shows that affinity-purified His-tagged BEN binds the EFG sequence. The proteins purified on a nickel column were run on 10% SDS/PAGE and electroblotted to a nitrocellulose membrane. The filters were probed with labeled EFG (lane 1) or UCD (lane 2) oligonucleotides, respectively. The single band around 90 kDa is the truncated His-tagged BEN fusion protein.