Figure 1.
(A) Transcriptional activation in a previously described E. coli-based genetic screen [developed by Hochschild and coworkers (8, 10)] for studying protein–DNA and protein–protein interactions. (B) Modified reporter template for our E. coli-based genetic selection system. (C) Model for transcriptional activation of the Pzif promoter by fusion proteins Gal11P-Zif123 and αGal4. ZF1, ZF2, and ZF3 are the three zinc fingers of the Zif268 protein. (Although Gal11P-Zif123 efficiently activates our Pzif promoter, we note that the spacing between the Zif268-binding site and the transcription start site has not yet been optimized.)