Figure 6.

Expression Constructs. The constructs were assembled from cloned or amplified DNA. All junctions were sequenced to confirm correct assembly. The green box represents the reporter EGFP-F. The maroon box represents a 300 bp fragment of the zebrafish BDNF gene that begins 280 bps upstream from exon 2 and ends at nucleotide 21 of the coding exon and thus contains the splice acceptor site. Note that the5' exons are directly joined to reporter in the shorter constructs. All constructs share the 3' UT and polyadenylation signal of the IE of SV40.