Figure 9.

Expression of BDNF 5' exons in juvenile zebrafish and skeletal muscle of adult female zebrafish. Total RNA was extracted from two whole juvenile zebrafish and skeletal muscle of an adult female zebrafish. The RNA was analyzed by two rounds of nested RT-PCR for the presence of exons 1a, 1b/b' and 1c. Both outside and inside primers spanned at least one intron. An aliquot of each cDNA preparation was diluted 1:100 and amplified with beta-actin primers to assess the quality of the cDNA preparations. The beta-actin primers span at least one intron. The 5' RACE clones were used as control templates and size markers. Amplified DNA was separated according to size by electrophoresis on a 1.8% agarose gel containing ethidium and visualized under UV light.