Skip to main content
. 2000 Jun 13;97(13):7423–7428. doi: 10.1073/pnas.120175097

Figure 2.

Figure 2

Activation of Btk and PKCβI depends on Syk. (A) Syk− or syk-reconstituted syk− RBL-2H3 cells were sensitized with anti-DNP IgE and stimulated by antigen. Immunoprecipitates with anti-Btk and anti-Lyn were subjected to autophosphorylation (Btk Kinase and Lyn Kinase). The same blots were probed with the respective antibodies (Blots). (B) Anti-PKCβI immunoprecipitates were prepared from similarly stimulated syk− or syk-reconstituted syk− RBL-2H3 cells and followed by autophosphorylating reactions (PKCβI Kinase). The same blot was probed with anti-PKCβI antibody (PKCβI Blot). Anti-JNK1 immunoprecipitates were subjected to kinase assays using GST-c-Jun (1–79) as substrate. Reaction products were analyzed by SDS/PAGE, electroblotting, and autoradiography (JNK1 Kinase). The same blot was probed with anti-anti-JNK1 antibody (Anti-JNK1 Blot). Syk deficiency of syk− cells was confirmed by immunoblotting of total cell lysates (Anti-Syk Blot).