Fig. 6. Bax undergoes a conformational change in response to Nbk expression. SW480 cells, Bax-negative DU145 mock transfectants and DU145-Bax clones 2, 3 and 4 were transduced with Ad5-myc-NBK-tTA and cultured for 24 h in the presence (Tet-off condition) or absence of vector and doxycyclin (Tet-on condition). Control cells were grown in the absence of doxycyclin. (A) Cells were stained with a conformation-specific antibody against the Bax N-terminus and analyzed by flow cytometry. The percentage of immunostained cells is indicated between markers. (B) Bar chart showing mean ± SD of cells expressing activated Bax. Data were obtained from three independent experiments.