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. 2000 May 30;97(13):7429–7434. doi: 10.1073/pnas.050578497

Figure 1.

Figure 1

Flow cytometric analysis of bone marrow cells. Cells were isolated from wild-type, 3-83Igi hemizygous (3-83Hi/+;3-83ki/+), and 3-83Igi homozygous (3-83Hi/Hi;3-83ki/ki) mice, all on a BALB/c background. Cells were stained for B220, IgM, and 3-83Ig (by the 54.1 antibody) in a three-color reaction. (Upper) B220 and IgM staining of cells in the lymphocyte gate. Numbers refer to the frequency of B220low/surface IgM (sIgM)− cells (region R3) in the total B220low cell population (region R2). (Lower) The 54.1 and IgM staining of B220low lymphocytes (R2 region in Upper). Numbers refer to frequency of 3-83Ig+ cells in the total sIgM+ (B220low) cell population.