Table I. Two-hybrid analysis demonstrating the binding of a Pbs2 docking site to the kinase domain of Ssk2 or Ssk22.
| Gal4 activationdomain construct | LexA DNA-binding domain construct |
|||
|---|---|---|---|---|
| RasV12 | Ssk1 | Pbs2(1–67) | Pbs2V54G(1–67) | |
| Raf | 6.2 | 1.4 | 0.3 | 0.3 |
| Ssk2(50–1579) | 0.3 | 248.6 | 0.5 | 0.5 |
| Ssk2(50–1148) | 0.4 | 203.7 | 0.7 | 0.4 |
| Ssk2(1128–1579) | 0.5 | 1.2 | 79.9 | 0.3 |
| Ssk22(62–1331) | 0.4 | 574.4 | 18.5 | 0.4 |
| Ssk22(998–1331) | 0.4 | 1.3 | 63.1 | 0.4 |
Values are arbitrary units of β-galactosidase activity (see Materials and methods), averaged from six assays (duplicate determinations with three independent transformants).