Figure 3.

Efficient antigen-dependent receptor editing in 3-83KIH-reconstituted bone marrow chimeras. Lethally radiated antigen-free B10.D2 (H-2^d) or antigen-carrying C57BL6 (H-2^b) mice were reconstituted with 3-83KIH bone marrow and analyzed 3 weeks later. (A) Bone marrow B cell expression of 3-83 Id and IgM (Upper). Expression of B220 and CD43 on gated, IgM⁻ cells (Lower); window shows frequencies of small pre-B type cells. (B) 3-83 sIg expression in the spleen of 3-83KIH-reconstituted chimeras in the presence (H-2^b) or absence (H-2^d) of antigen. Spleen cells were stained with anti-Id and anti-IgM mAbs. The percentage of IgM⁺ Id⁻ mature B cells was calculated on the lymphocyte-gated population. Data are representative of three independent experiments each containing two individual mice per group. (C) Ratio of the frequencies of λ⁺ and κ⁺ B cells in the spleens of the indicated mice as determined by flow cytometry. Each point represents data from an individual mouse.