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Immunoglobulin expression in hybridomas from 3-83KI spleen fusion
| Ig expressed | No. positive hybrids/total tested | |
|---|---|---|
| H-2d | H-2b/d | |
| IgMa | ND | 24/24 |
| 3-83 Id+ | 4/93 | 0/136 |
| κ | 51/93 | 11/136 |
| λ | 42/93 | 125/136 |
L-chain usage and 3-83 secretion were assessed by ELISA. 3-83 idiotype was assessed with mAb 54.1. ND, not determined.
