Fig. 5. Effect of PDE2 overexpression on superoxide concentration and RSV in RAS2^val19 and wild-type cells. (A) In situ superoxide ion concentrations detected by DHE staining in wild-type and RAS2^val19 cells with (+) and without (–) Pde2p overproduction. (B) RSV analysis of RAS2^val19 cells carrying either the empty vector (solid red line) or overexpressing PDE2 (dotted red line). The RSV analysis of a bcy1 mutant is shown by the black line. See Materials and methods for details of the procedures. (C) Glycogen content (inset) and heat sensitivity (shift from 30°C to 52°C) of wild type (open squares), RAS2^val19 cells (open cicrcles) and bcy1 mutants (filled squares).