Table 1.
ELISA of secreted cytokines
| Cytokine, pgr/ml | Resting state
|
Stimulation with MBP
|
Stimulation with Cop-1
|
Stimulation with Con A
|
||||
|---|---|---|---|---|---|---|---|---|
| Tmbp | Tcop | Tmbp | Tcop | Tmbp | Tcop | Tmbp | Tcop | |
| IFN-γ | 725 | 6,645 | 15,692 | 925 | 7,242 | 11,825 | 22,758 | 22,525 |
| IL-10 | 41 | 382 | 1,941 | 13 | 365 | 7,244 | 3,565 | 6,503 |
Supernatants from unstimulated T cells or T cells stimulated for 48 h with Con A mitogen, MBP antigen, or Cop-1 antigen in stimulation medium were subjected to sandwich ELISA. The table shows the concentration of cytokines. The amounts of secreted T helper 2-related cytokines were significantly higher in supernatants of Cop-1-reactive T cells than in supernatants of T cells specific to MBP.