Fig. 7. Ugt51 is not involved in starvation-induced macroautophagy. (A) Light microscopy of P.pastoris wild-type, ugt51Δ, and starvation-induced macroautophagy-deficient gsa11/paz7 cells after 8 h incubation in glucose medium lacking nitrogen. In wild-type and ugt51Δ cells, black granules representing macroautophagic bodies were visible in the vacuolar lumen, but not in the gsa11 cells. (B) Cell viability was measured after 10 day incubation in glucose medium lacking nitrogen. The value (%) represents colony forming ability with reference to the wild type. Both gsa7 and gsa11 cells are macroautophagy deficient.