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. 2003 Jul 1;22(13):3231–3241. doi: 10.1093/emboj/cdg331

Table I. Pichia pastoris strains used in this study.

Strain Designation Genotype (explanation, plasmid used for transformation) Reference
PPY12 wild-type arg4 his4 (parental strain for UGT51 deletion) Sakai et al. (1998)
STW1 GFP–PTS1WT PPY12 his4::PAOX1GFP-PTS1-HIS4 (GFP targeted to peroxisomes) Sakai et al. (1998)
PZR7 paz7Δ STW1 paz7::ZeocinR Mukaiyama et al. (2002)
PZR12 paz12Δ STW1 paz12::ZeocinR Mukaiyama et al. (2002)
PZO101 ugt51Δ PPY12 ugt51::ZeocinR (sterol glucosyltransferase KO-strain) This study
PZO102 PoUGT51 PZO101 his4::PUGT51UGT51-HIS4 (pEAE) This study
PZO103 GFP-PTS1ugt51Δ PZO101 his4::PAOX1GFP-PTS1-HIS4 (pTW51) This study
PZO104 ugt51Δpep4prb1 SMD1163 (pep4 prb1 his4) his4::pTW51 ugt51::Zeocin This study
PZO105 PoUGT51-PHΔ PZO101 his4::PUGT51UGT51-PHΔ -HIS4 (pPZ4dPH) This study
PZO200 PoGFP-UGT51 PZO101 his4::PUGT51GFP-UGT51-HIS4 (pGPZ43) This study
PZO201 GFPUGT51 PZO101 his4::PAOX1GFP-UGT51-HIS4 (pGPZ42) This study
PZO202 GFPUGT51-PHΔ PZO101 his4::PAOX1GFP-UGT51-PHΔ-HIS4 (pGPZ4dPH) This study
PZO203 GFPUGT51-GRAMΔ PZO101 his4::PAOX1GFP-UGT51-GRAMΔ-HIS4 (pGPZ4dGRAM) This study
PZO204 GFPUGT51-CATΔ PZO101 his4::PAOX1GFP-UGT51-CATΔ-HIS4 (pGPZ4dCT) This study
PZO205 GFPUGT51-Y642P PZO101 his4::PAOX1GFP-UGT51-Y642P-HIS4 (pGPZ4Y642P) This study
PZO206 GFP–UGT51/HA-Paz2 PZO201 arg4::PPAZ2HA-PAZ2-ARG4 This study