Table 1.

rr5 disrupts a gene required during early larval development

Time (h)	Stage	Adult animals after up-shift (%)
8	L1	0.0 ± 0.0
16	L1	0.6 ± 1.1
24	L1	2.1 ± 1.0
32	L2	8.7 ± 1.3
40	L2	40.1 ± 0.5
48	L3	47.1 ± 5.8
56	L3	75.4 ± 3.1
64	L3	96.6 ± 0.5
72	L4	100 ± 0.0

rr5 mutant embryos maintained at 15°C were hatched in M9 buffer and then cultured at 15°C. At 8-h intervals, 50 worms were upshifted to restrictive temperature (25°C), and animals that reached the adult stage after 48 h in culture were scored. Results are expressed as percentage of the total worm population present on the plate. All progeny derived from upshifted rr5 animals that grew to adulthood at 25°C successfully terminated embryogenesis before recapitulating the L1 arrest phenotype typical of rr5 at 25°C.