Table 2.
inx-6(rr5) reduces pharyngeal contraction frequency but does not affect the capacity to respond to serotonin (5HT)
|
Pumping rate (contractions/min)
|
|||
|---|---|---|---|
| Genotype | -5HT | +5HT | |
| 15°C | N2 | 134.4 ± 11.2 | 149.3 ± 16.6 |
| inx-6(rr5) | 131.9 ± 12.1 | 141.2 ± 15.1 | |
| eat-2(ad465) | 17.9 ± 4.6 | 16.9 ± 4.6 | |
| 25°C | N2 | 165.8 ± 15.3 | 182.6 ± 15.1 |
| inx-6(rr5) | 76.8 ± 12.3 | 103.6 ± 16.2 | |
| eat-2(ad465) | 16.6 ± 5.1 | 17.4 ± 5.8 | |
Wild-type N2 animals were used as positive control. All animals were examined at the L1 stage by mounting on 2% agarose pads with M9 buffer with (+5HT) or without (-5HT) 5 mM serotonin. The pumping rate was monitored using a compound microscope by counting the rhythmic motion of the grinder in the terminal bulb of individual animals over the course of 1 min beginning immediately after location of the specimens and adjustment of focus. Values are expressed as contractions/min ± SD observed from 20 different animals (n = 20).