Table 3.

eat-5 can partially rescue the inx-6(rr5) mutation

	Distribution of worms at each postembryonic stage after 40 h at 25°C (%)
Genotype	L1	L2	L3	L4	Adult
inx-6; Ex[inx-6::GFP]a	99	1	0	0	0
inx-6; Ex[inx-6::INX-6; inx-6::GFP]b	0	0	0	0	100
inx-6; Ex[inx-6::EAT-5; inx-6::GFP]	46	31	16	5	2

Expression of eat-5 under the control of the inx-6 promoter can rescue the inx-6(rr5) mutation although the rescued animals are compromised in growth rate. To identify transgenic animals at the L1 stage, the nonrescuing inx-6 transcriptional fusion with GFP was used as the cotransformation marker: inx-6(rr5) mutants that possess a wild-type inx-6 transgene (inx-6::INX-6) were used as a positive control. inx-6(rr5) mutants that carried the nonrescuing inx-6::GFP transcriptional fusion reporter were used as a negative control. L1 stage animals (100) from each strain were picked and maintained on food at 25°C for analysis. The distribution of animals at each stage was scored after 40 h, when all the animals in the positive control had reached the adult stage. More than 59% of the mutant strain inx-6; Ex[inx-6::EAT-5; inx-6::GFP] reached the adult stage only after 88 h (our unpublished data).

^ainx-6; Ex[inx-6::GFP] animals behave identically to inx-6(rr5) mutants. Most animals (>98%) did not progress beyond the L1 stage even after 88 h when maintained at 25°C.

^binx-6; Ex[inx-6::INX-6; inx-6::GFP] is a fully rescued inx-6(rr5) mutant strain and it grows and develops as wild-type animals.