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. 2003 Jul;14(7):2665–2676. doi: 10.1091/mbc.E02-12-0832

Figure 1.

Figure 1.

Infusion of soluble laminin-1 disrupts endogenous laminin in the wild-type mouse hippocampus but is not neurotoxic. Wild-type mice were unilaterally infused with buffer or purified mouse laminin-1 (0.5 mg/ml) for 7 d, brain sections were prepared and stained with anti-laminin-1 antibody. Laminin in the hippocampal neuronal call layers in buffer infused animals is intact (A), but in the soluble laminin-infused animals the endogenous laminin in the CA 1 and DG neuronal cell layers of the infused side is disrupted (B). Cresyl violet (C) and Fluoro Jade B (E) staining show that infusion of soluble laminin is not neurotoxic. Higher magnification of the boxed area in C is shown in D. (F) A positive control for Fluoro Jade B staining, which stains degenerating neurons after kainate injection. CA1, CA3, and DG denote regions of the hippocampus. Ln, Laminin-1 immunostaining; CV, cresyl violet staining; FJB, Fluoro Jade B staining. Bar in C for A–C, 500 μm; bar in D for D–F, 25 μm.